LR-15

General

Cell Line

hPSCreg name ICGi043-C
Cite as:
ICGi043-C (RRID:CVCL_C3RY)
Alternative name(s)
LR-15
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
ICGi043-A
(LR-21)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson's Disease
ICGi043-B
(LR-2)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson's Disease
STBCi004-B-1
(SFC832-03-06 LRRK2WT/WT C47)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
ICGi042-A
(PD12-4Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson's Disease
ICGi042-B
(PD12-5Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson's Disease
ICGi042-C
(PD12-6Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson's Disease
ICGi023-A
(PD45-6-1Lm)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson's Disease
STBCi004-B
(SFC832-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi004-C
(SFC832-03-07)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
SHEHDNi002-A
(KY03AP)
Donor's gene variants:
LRRK2, DNAJC6
Donor diseases:
Parkinson's Disease
UOXFi007-A
(MK002-4)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi007-B
(MK002-6)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi007-C
(MK002-7)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-A
(MK144-1)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-B
(MK144-7)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-C
(MK144-10)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi004-A
(SFC832-03-19)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
DANi011-A
(LRRK2-011-C1)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson's Disease
ESi006-A
(SP13#4)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi005-B
(SFC833-03-05)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi005-C
(SFC833-03-14)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-B
(SFC855-03-08)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-C
(SFC855-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi005-A
(SFC833-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-A
(SFC855-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
DANi005-A
(LRRK2-GBA-005-C1)
Donor's gene variants:
GBA, LRRK2
Donor diseases:
Parkinson's Disease
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-4
(AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
MPIi003-A-1
(IM2GC, L2-2GC)
Donor diseases:
obsolete_Parkinson's disease
EDi001-B-1
(AST18-7A)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-B-2
(AST18-7B)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-B-3
(AST18-5D)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-B-4
(AST18-6A)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-A-5
(AST23-2KO-II8B)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
LCSBi009-A-1
(RHOT1_R272Q_clone18_IsogenicControl)
Donor diseases:
Parkinson's Disease
LCSBi010-A-1
(RHOT1_R450C_clone6_IsogenicControl)
Donor diseases:
Parkinson's Disease
LCSBi010-A-2
(RHOT1_R450C_clone10_IsogenicControl)
Donor diseases:
Parkinson's Disease
LCSBi012-A-1
(RHOT1_T610A_clone62.19.37_IsogenicControl)
Donor diseases:
Parkinson's Disease
LCSBi008-A-1
(delP GC13, DJ-1-delP GC13)
Donor diseases:
Parkinson's Disease
PNUSCRi004-A
(GBA PD iPSC8)
Donor diseases:
Parkinson's Disease
JUCGRMi002-A
(DupA5)
Donor diseases:
Parkinson's Disease
JUCGRMi002-B
(DupA13)
Donor diseases:
Parkinson's Disease
JUCGRMi002-C
(DupA18)
Donor diseases:
Parkinson's Disease
JUCGRMi003-A
(PH13)
Donor diseases:
Parkinson's Disease
SUSMi005-A-1
(SNCA3X 0KO C1, SNCA3X 0KO C2)
Donor diseases:
obsolete_Parkinson's disease
Last update 5th December 2022
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Provider

Generator Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Owner Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Distributors
Derivation country Russia

External Databases

BioSamples SAMEA111344465
Cellosaurus CVCL_C3RY
Wikidata Q117704516

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 70-74
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Clinical diagnosis: LRRK2-associated Parkinson's disease, tremulous form, stage 2.0 on the Hoehn and Yahr scale, with predominant involvement of the right limbs, complicated by motor fluctuations (single dose depletion phenomenon, morning akinesia), mild postural deformity with developing camptocormia, mild mental disorders. Disease duration: 5 years
The donor is a carrier of a disease-associated mutation and affected.
Stage
Stage 2.0 on the Hoehn and Yahr scale
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic variants
LRRK2 (target)
12q12
NM_198578.4(LRRK2):c.6055G>A
NP_940980.4:p.Gly2019Ser
Heterozygous
VCV000001940.40
Family history Father's cousin had a tremor

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA112177268

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? No
a non-profit company? No
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does consent permit access to medical records of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? N.P. Behtereva Institute of the Human Brain of the Russian Academy of Sciences
Approval number Protocol number 1, 26/11/2020
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Addgene
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
Blood drawn from a limb.
Synonyms
  • Peripheral Blood
  • Peripheral blood
Age of donor (at collection) 70-74
Collected in 2020
Passage number reprogrammed 1

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones The selection of colonies was carried out according to morphological criteria. We selected flat monolayer colonies with tightly packed cells with high nuclear/cytoplasmic ratio. Embryonic stem cell-like clones were picked by a glass microcapillary.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Gelatin
Feeder cells Mouse embryonic fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knock-out DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
GlutaMAX 2 mM
NEAA 0.1 mM
2-mercaptoethanol 0.1 mM
Penicillin-Streptomy 100 U/ml
rhFGF basic 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
NANOG
Yes
Alkaline Phosphatase
Yes
Morphology pictures
Morphol LR-15 4p.tif
Morphology of ICGi043-C iPSC at passage 4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CK18
Yes
SOX17
Yes
Morphology
Sp dif LR-15 9p KRT18_r SOX17_g.tif
Immunofluorescent analysis for endoderm markers SOX17 (green signal) and KRT18 (red signal) in ICGi043-C iPSC at passage 9. DAPI (blue signal)
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Actin, alpha 2, smooth muscle, aorta
Yes
CD31
Yes
Morphology
Sp dif LR-15 9p aSMA_r+DAPI.tif
Immunofluorescent analysis for mesoderm marker alfa SMA (red signal) in ICGi043-C iPSC at passage 9. DAPI (blue signal)
Sp dif LR-15 9p CD31_r.tif
Immunofluorescent analysis for mesoderm marker CD31 (red signal) in ICGi043-C iPSC at passage 9. DAPI (blue signal)
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes
GFAP
Yes
Morphology
Sp dif LR-15 9p bIII_r+DAPI.tif
Immunofluorescent analysis for ectoderm marker TUBB3 (red signel) in ICGi043-C iPSC at passage 9. DAPI (blue signal)
Sp dif LR-15 9p GFAP_g+DAPI.tif
Immunofluorescent analysis for ectoderm marker GFAP (green signal) in ICGi043-C iPSC at passage 9. DAPI (blue signal)

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotyping and G-banding show ICGi043-C iPSCs have a normal 46, XX karyotype at passage 5.
Passage number: 5
Karyotyping method: G-Banding

Other Genotyping (Cell Line)