SHEHDNi002-A

KY03AP

General

Cell Line

hPSCreg name SHEHDNi002-A
Cite as:
SHEHDNi002-A (RRID:CVCL_A3BX)
Alternative name(s)
KY03AP
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi004-B-1
(SFC832-03-06 LRRK2WT/WT C47)
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LRRK2
Donor diseases:
Parkinson disease
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LRRK2
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UOXFi008-A
(MK144-1)
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LRRK2
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LRRK2
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STBCi004-C
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Parkinson disease
UOXFi007-B
(MK002-6)
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LRRK2
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Parkinson disease
UOXFi007-C
(MK002-7)
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LRRK2
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Parkinson disease
UOXFi008-B
(MK144-7)
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LRRK2
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Parkinson disease
UOXFi008-C
(MK144-10)
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LRRK2
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Parkinson disease
STBCi004-A
(SFC832-03-19)
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LRRK2
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Parkinson disease
DANi011-A
(LRRK2-011-C1)
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LRRK2
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ESi006-A
(SP13#4)
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LRRK2
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Parkinson disease
STBCi005-B
(SFC833-03-05)
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LRRK2
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Parkinson disease
STBCi005-C
(SFC833-03-14)
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LRRK2
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STBCi007-B
(SFC855-03-08)
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LRRK2
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STBCi007-C
(SFC855-03-01)
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LRRK2
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ICGi043-A
(LR-21)
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LRRK2
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Parkinson Disease
ICGi043-B
(LR-2)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ICGi043-C
(LR-15)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ICGi023-A
(PD45-6-1Lm)
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LRRK2
Donor diseases:
Parkinson Disease
STBCi005-A
(SFC833-03-01)
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LRRK2
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Parkinson disease
STBCi007-A
(SFC855-03-06)
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LRRK2
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DANi005-A
(LRRK2-GBA-005-C1)
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GBA, LRRK2
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Parkinson Disease
ICGi042-A
(PD12-4Lm)
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LRRK2, PINK1
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Parkinson Disease
ICGi042-B
(PD12-5Lm)
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LRRK2, PINK1
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Parkinson Disease
ICGi042-C
(PD12-6Lm)
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LRRK2, PINK1
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Parkinson Disease
EDi001-A-1
(AST22-C, AST23-C)
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SNCA, SNCA
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Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
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SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-4
(AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6)
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SNCA, SNCA, SNCA, SNCA
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Parkinson disease
MPIi003-A-1
(IM2GC, L2-2GC)
Donor diseases:
obsolete_Parkinson's disease
EDi001-B-1
(AST18-7A)
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SNCA
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Parkinson disease
EDi001-B-2
(AST18-7B)
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SNCA
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Parkinson disease
EDi001-B-3
(AST18-5D)
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SNCA
Donor diseases:
Parkinson disease
EDi001-B-4
(AST18-6A)
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SNCA
Donor diseases:
Parkinson disease
EDi001-A-5
(AST23-2KO-II8B)
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SNCA
Donor diseases:
Parkinson disease
LCSBi009-A-1
(RHOT1_R272Q_clone18_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi010-A-1
(RHOT1_R450C_clone6_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi010-A-2
(RHOT1_R450C_clone10_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi012-A-1
(RHOT1_T610A_clone62.19.37_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi008-A-1
(delP GC13, DJ-1-delP GC13)
Donor diseases:
Parkinson Disease
EDi001-A
(AST22, AST23, SAMEA3319992)
Donor's gene variants:
SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
UOXFi001-A
(MK071-1)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-B
(MK071-3)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-C
(MK071-5)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-D
(MK071-6)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
EDi001-B
(AST18)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
SUSMi005-A-1
(SNCA3X 0KO C1, SNCA3X 0KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-2
(SNCA3X 1KO C2, SNCA3X 1KO C1)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-3
(SNCA3X 2KO C1, SNCA3X 2KO C2)
Donor diseases:
obsolete_Parkinson's disease
Last update 4th March 2021
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Provider

Generator Department of Neurology (SHEHDN)
Owner Department of Neurology (SHEHDN)
Distributors
Derivation country China

External Databases

BioSamples SAMEA7895017
Cellosaurus CVCL_A3BX
Wikidata Q105511072

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Ethnicity Han nationality

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic variants
LRRK2 (target)
12q12
NM_198578.4(LRRK2): c.7153G>A
XP_005268686.1:p.Gly2385Arg
Heterozygous
DNAJC6 (target)
NM_001256864.2(DNAJC6): c.1577A>G
:p.Asn526Ser
Heterozygous

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA8359743

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Shanghai East Hospital
Approval number EC. D(BG).016.01.1
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Any skin fibroblast that is part of some dermis.
Source cell origin
The portion of the upper extremity between the shoulder and the elbow. For clinical purposes this term is also used to refer to the whole superior limb.
Synonyms
  • ARM
  • Arm
  • Brachium
  • Upper Arm
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
SSEA-4
Yes
NANOG
Yes
TRA 1-81
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
When the iPSCs were approximately 70% confluent, the cells were harvested and plated for differentiation per the STEMdiffTM Trilineage Differentiation Kit protocol (Stem Cell Technologies). Five to seven days, the cells were fixed with 4% PFA for IF staining of the lineage-specific markers, Nestin (ectoderm), SMA (mesoderm), and AFP (endoderm).
Method documentation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 20
Karyotyping method: G-Banding

Other Genotyping (Cell Line)