PINK1-I368N-C2
The cell line is not validated yet.
GIBHi003-A
General
Cell Line |
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| hPSCreg name | GIBHi003-A |
| Cite as: | GIBHi003-A (RRID:CVCL_YC26) |
| Alternative name(s) |
PINK1-I368N-C2
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
LCSBi002-B (ND40066-clone 7, ND40066-PINK1/PARK6-ILE368ASN-clone 7) Donor's gene variants: PINK1, PINK1 Donor diseases: Parkinson's Disease LCSBi002-C (ND40066-clone 8, ND40066-PINK1/PARK6-ILE368ASN-clone 8) Donor's gene variants: PINK1, PINK1 Donor diseases: Parkinson's Disease EDi001-A-2 (AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-3 (AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-4 (AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease STBCi004-B-1 (SFC832-03-06 LRRK2WT/WT C47) Donor's gene variants: LRRK2 Donor diseases: Parkinson disease |
| Last update | 17th October 2019 |
| User feedback | |
Provider |
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| Generator | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences (GIBH) |
| Owner | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences (GIBH) |
| Distributors | |
| Derivation country | China |
External Databases |
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| BioSamples | SAMEA6114046 |
| Cellosaurus | CVCL_YC26 |
| Wikidata | Q93561145 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
| Family history | Yes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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External Databases (Donor) |
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| BioSamples | SAMEA6114047 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | No |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | Coriell Institute |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | The ethics committee of the Guangzhou Institutes of Biomedicine and Health |
| Approval number | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | The ethics committee of the Guangzhou Institutes of Biomedicine and Health |
| Approval number | |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Synonyms
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| Source cell line lot number |
ND40066
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| Source cell line vendor | Coriell |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Medium |
mTeSR™ 1
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| GATA3 |
Yes |
| GATA6 |
Yes |
| RXRG |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| CDX2 |
Yes |
| HAND1 |
Yes |
| SNAI2 |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| MAP2 |
Yes |
| PAX6 |
Yes |
| SOX1 |
Yes |
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY
Passage number: 15
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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