HEBHMUi001-A

General#

Cell Line

hPSCreg Name HEBHMUi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th March 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Hebei Medical University (HEBHMU)
Owner Hebei Medical University (HEBHMU)
Distributors
Derivation country China

External Databases

BioSamples SAMEA5574028

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson's Disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Disease associated phenotypes
  • behavior
Family history no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5574029

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
Fibroblasts
Source cell origin
Fibroblasts
Source cell line lot number
HEBHMUi001-A
Age of donor (at collection) 60-64
Collected in 2018

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Yes
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Self-renewal
Unkown
Endoderm
Unkown
Mesoderm
Unkown
Ectoderm score
Positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)