Reprogramming of one human induced pluripotent stem cell line from healthy donor
In this study, skin biopsy was, and the fibroblasts were isolated from the dermal explant cultures. Human induced pluripotent stem cell (iPSC) line was generated from the skin fibroblasts collected from a healthy 50-year old male donor with informed consent. The reprogramming of fibroblasts was performed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc. The generated iPSCs were confirmed integration-free, expressed pluripotency markers, displayed the normal karyotype, and demonstrated trilineage differentiation potential. This iPSC model can be used to model physiological processes and screen drug validation in vitro. Copyright © 2021. Published by Elsevier B.V.
|Authors||Cao S, Ma J, Zhang J, Guo R, Liu X, Li M|
|Journal||Stem cell research|
|Publication Date||2021 Dec 1;57:102613|