RHOT1_R272Q_clone1_PD

General

Cell Line

hPSCreg name LCSBi009-A
Cite as:
LCSBi009-A (RRID:CVCL_C8G3)
Alternative name(s)
RHOT1_R272Q_clone1_PD
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 8th April 2023
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Provider

Generator Luxembourg Centre for Systems Biomedicine (LCSB)
Owner Luxembourg Centre for Systems Biomedicine (LCSB)
Distributors
Derivation country Luxembourg

External Databases

BioSamples SAMEA112885173
Cellosaurus CVCL_C8G3
Wikidata Q123032932

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Align with cell line owner for Material Transfert agreement
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclones

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA112885174

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink rejko.krueger@uni.lu / rejko.krueger@lih.lu
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? CNER Comité National d'Ethique et de Recherche
Approval number 201411/05
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type

Reprogramming method

Vector type Non-integrating
Vector in vitro transcribed RNA replicon expressing the reprogramming factors in a single self-replicating polycistronic transcript
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Brachiury
Yes
Morphology
iPSCs were plated on Matrigel-coated coverslips two days before the in vitro differentiation procedure started. The Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, Cat No. SC027B) was used to verify iPSCs capacity to differentiate into the three germ layers. ICC of the ectodermal marker OTX2, the mesodermal marker Brachyury and the endodermal marker SOX17 was performed. Nuclei were stained with Hoechst and images were acquired using a Zeiss AxioObserverZ1 microscope.
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
OTX2
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)