RHOT1_R272Q_clone18_IsogenicControl
LCSBi009-A-1
General
Cell Line |
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| hPSCreg Name | LCSBi009-A-1 |
| Alternative name(s) |
RHOT1_R272Q_clone18_IsogenicControl
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 8th April 2023 |
| User feedback | |
Provider |
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| Generator | Luxembourg Centre for Systems Biomedicine (LCSB) |
External Databases |
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| BioSamples | SAMEA112885175 |
| CLO | CLO_0104715 |
| Cellosaurus | CVCL_C8G4 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in:
Align with cell line owner for Material Transfert agreement
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
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External Databases (Donor) |
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| BioSamples | SAMEA112885174 |
Ethics
Also have a look at the ethics information for the parental line
LCSBi009-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
LCSBi009-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | in vitro transcribed RNA replicon expressing the reprogramming factors in a single self-replicating polycistronic transcript |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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Morphology pictures
Typical iPSC morphology
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
female
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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