LCSBi009-A-1

RHOT1_R272Q_clone18_IsogenicControl

General

Cell Line

hPSCreg Name
LCSBi009-A-1
Alternative name(s)
RHOT1_R272Q_clone18_IsogenicControl
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th April 2023
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Provider

Generator Luxembourg Centre for Systems Biomedicine (LCSB)

External Databases

BioSamples SAMEA112885175
CLO CLO_0104715
Cellosaurus CVCL_C8G4

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Align with cell line owner for Material Transfert agreement
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson's Disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA112885174

Ethics

Also have a look at the ethics information for the parental line LCSBi009-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line LCSBi009-A.

Reprogramming method

Vector type Non-integrating
Vector in vitro transcribed RNA replicon expressing the reprogramming factors in a single self-replicating polycistronic transcript
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
Morphology pictures
Typical iPSC morphology
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Brachiury
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
OTX2
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Parkinson's Disease
This line has been corrected, thus is used as an isogenic control of the Parkinson's disease patient-derived line LCSBi009-A
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic modifications
RHOT1 (target)
Isogenic modification
Homozygous
Correction of the point mutation via Crispr-Cas9
Repaired