Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor


In this study, skin biopsy was collected from a healthy 48-year old male donor with informed consent, and the fibroblasts were isolated from the dermal explant cultures. Here, a human induced pluripotent stem cell (iPSC) line was derived from the fibroblasts using the reprogramming four Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc). The generated iPSCs were integration-free, displayed the normal karyotype, expressed pluripotency markers and demonstrated trilineage differentiation potential in vitro. This iPSC model will be useful for investigating physiological processes, drug validation as well as a control in pathological mechanistic studies. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Guo R, Mu W, Liu X, Zhang J, Liu B, Du X, He J, Ma J, Cui H
Journal Stem cell research
Publication Date 2021 May;53:102339
PubMed 33892291
DOI 10.1016/j.scr.2021.102339

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