PD-OPA1-A495V#72

General#

Cell Line

hPSCreg Name OSRi001-A
Alternative name(s)
PD-OPA1-A495V#72
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator Ospedale San Raffaele (OSR)

External Databases

BioSamples SAMEA5712221

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson's Disease
The donor is affected.
Stage
Late stage
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease

External Databases (Donor)

BioSamples SAMEA5712222

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Prof. Valerio Carelli, IRCCS Institute of Neurological Sciences of Bologna (ISNB), Bellaria Hospital, Bologna
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink Prof. Valerio Carelli, IRCCS Institute of Neurological Sciences of Bologna (ISNB), Bellaria Hospital, Bologna
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Policy for access to genetic information Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Comitato Etico dell'Azienda Ospedaliera-Universitaria di Bologna
Approval number Prot. 123/2006
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Comitato Etico dell'Azienda Ospedaliera-Universitaria di Bologna
Approval number Prot. 123/2006
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Fibroblasts
Source cell origin
Muscle of arm
Synonyms
  • Arm muscle

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium mTeSR™ 2

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Smooth Muscle Cell
Ont Id: CL_0000192
In vitro spontaneous differentiation
Neuroepithelial Stem Cell
Ont Id: CL_0002259
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)