GBA-003-C8

General

Cell Line

hPSCreg name DANi003-H
Cite as:
DANi003-H (RRID:CVCL_XJ39)
Alternative name(s)
GBA-003-C8
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
DANi002-C
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GBA
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Parkinson's Disease
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GBA, LRRK2
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LCPHi001-A
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GBA
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obsolete_Parkinson's disease
DANi006-F
(GBA-006-C6)
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GBA
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DANi010-A
(GBA-010-C1)
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GBA
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PNUSCRi001-A
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GBA
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(GBA PD iPSC9)
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UOXFi002-A
(MK082-26)
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GBA1
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UOXFi002-B
(MK082-30)
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GBA1
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UOXFi003-A
(MK088-1)
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(MK088-7)
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STBCi025-A
(SFC834-03-01)
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Parkinson disease
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(SFC834-03-03)
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GBA1
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Parkinson disease
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(SFC834-03-10)
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STBCi042-A
(SFC848-03-02)
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GBA1
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Parkinson disease
STBCi042-B
(SFC848-03-04)
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GBA1
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Parkinson disease
STBCi042-C
(SFC848-03-06)
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GBA1
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Parkinson disease
STBCi085-A
(SFC866-03-06)
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GBA1
Donor diseases:
Parkinson disease
STBCi085-B
(SFC866-03-02)
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GBA1
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Parkinson disease
STBCi085-C
(SFC866-03-05)
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GBA1
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STBCi088-A
(SFC872-03-05)
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GBA1
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Parkinson disease
STBCi088-B
(SFC872-03-02)
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(SFC872-03-03)
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GBA1
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UOXFi001-A
(MK071-1)
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GBA1
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Parkinson disease
UOXFi001-B
(MK071-3)
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GBA1
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Parkinson disease
UOXFi001-C
(MK071-5)
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GBA1
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Parkinson disease
UOXFi001-D
(MK071-6)
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GBA1
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Parkinson disease
STBCi084-A
(SFC871-03-12)
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(SFC871-03-04)
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GBA1
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Parkinson disease
CBIGi002-A
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GBA, GBA
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CBIGi002-A-1
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GBA, GBA
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UMi035-A
(GM07968)
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Gaucher disease type 1
LCSBi012-A-1
(RHOT1_T610A_clone62.19.37_IsogenicControl)
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Parkinson's Disease
LCSBi008-A-1
(delP GC13, DJ-1-delP GC13)
Donor diseases:
Parkinson's Disease
SUSMi005-A-1
(SNCA3X 0KO C1, SNCA3X 0KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-2
(SNCA3X 1KO C2, SNCA3X 1KO C1)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-3
(SNCA3X 2KO C1, SNCA3X 2KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-4
(SNCA3X 3KO C1, SNCA3X 3KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-5
(SNCA3X 4KO C1, SNCA3X 4KO C2)
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obsolete_Parkinson's disease
LCSBi011-A-1
(RHOT1_T351A_clone25.2_IsogenicControl)
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Parkinson's Disease
ESi004-A
([GD]FiPS-4F-21c)
Donor's gene variants:
GBA1
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Gaucher disease
LCSBi001-A
(VPS35 1_2)
Donor diseases:
obsolete_Parkinson's disease
LCSBi013-A
(GL2)
Donor diseases:
Parkinson's Disease
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-4
(AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
MPIi003-A-1
(IM2GC, L2-2GC)
Donor diseases:
obsolete_Parkinson's disease
EDi001-B-1
(AST18-7A)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
Last update 10th July 2019
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Provider

Generator Danish Research Institute of Translational Neuroscience (DAN)
Owner Danish Research Institute of Translational Neuroscience (DAN)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA5912471
Cellosaurus CVCL_XJ39
Wikidata Q93503203

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Stage
age of onset, 45
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic variants
GBA (target)
NM_001005741.2(GBA):c.1226A>G (p.Asn409Ser)
NP_000148.2:p.Asn409Ser
Heterozygous
DANi-003H Molecular analysis-v9b.tif
Sanger sequencing across GBA:c.1226A>G region
Is clinical information available? age of onset 45

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5912472

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink https://www.hih-tuebingen.de/ueber-uns/core-facilities/biobank/researchers/
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethik-Kommission an der Medizinischen Fakultät der Eberhard-Karls-Universität und am Universitätsklinikum Tübingen
Approval number 199/2011BO1
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Source cell line vendor Hertie biobank

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium TeSR™ E8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-81
Yes
Marker Present Absent
mCpG
OCT4
Method documentation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Morphology

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 41
Karyotyping method: G-Banding

Other Genotyping (Cell Line)