CBIGi002-A-1

GBA W378G-correction/2890, 2890-iso

General

Cell Line

hPSCreg Name
CBIGi002-A-1
Alternative name(s)
GBA W378G-correction/2890, 2890-iso
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th April 2022
Notes Isogenic control for CBIGi002-A.
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Provider

Generator Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG)
Derivation country Canada

External Databases

BioSamples SAMEA13204586
CLO CLO_0103795
Cellosaurus CVCL_C0K7
Wikidata Q112929371

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson's Disease
The donor is a carrier of a disease-associated mutation.
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic variants
GBA (target)
1q21
NM_000157.4:c.1249T>G+/-
NP_000148.2:p.Trp417Gly+/- (also known as p.Trp378Gly)
Heterozygous

External Databases (Donor)

BioSamples SAMEA13200845

Ethics

Also have a look at the ethics information for the parental line CBIGi002-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line CBIGi002-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
MIXL1
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
Pax6
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Isogenic modification
1q21
Heterozygous
After CRISP/Cas9 editing, Sanger sequencing confirmed that the wild-type TGG codon replaced the mutant GGG codon, correcting the heterozygous W378G missense mutation in CBIGi002-A.
Repaired