STBCi026-A-1

SFC840-03-03 LRRK2-/-D10

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
STBCi026-A-1
Alternative name(s)
SFC840-03-03 LRRK2-/-D10
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th May 2022
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Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA5859479
CLO CLO_0102948
Cellosaurus CVCL_A8Z8
Wikidata Q102114944

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493661

Ethics

Also have a look at the ethics information for the parental line STBCi026-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line STBCi026-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1

Characterisation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotype abnormalities: none compared to fibroblasts
Passage number: 31
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Parkinson disease
Genetic modifications
LRRK2 (target)
Gene knock-out
12q12
CRISPR/Cas9-mediated disruption of exon 3 and clone-screening for bi-allelic out-of-frame repair, to create Lrrk2 knockout line
CRISPR-associated (CRISPR/Cas) System