STBCi026-A-4

SFC840-03-03-PITX3-GFP

General#

Cell Line

hPSCreg Name
STBCi026-A-4
Alternative name(s)
SFC840-03-03-PITX3-GFP
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 13th April 2021
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Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA5859482
ECACC 66541208
CLO CLO_0103204
Cellosaurus CVCL_A4YP

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493661

Ethics#

Also have a look at the ethics information for the parental line STBCi026-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line STBCi026-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1

Characterisation#

Differentiation Potency
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
TUJ-1
Yes
PITX3
Yes
Morphology
Dopaminergic neuronal differentiation

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
PITX3 (target)
Gene knock-in
10q24.32
GFP
10q24.32
PITX3-GFP tag inserted, single copy.
CRISPR-associated (CRISPR/Cas) System