STBCi026-A-4

SFC840-03-03-PITX3-GFP

General

Cell Line

hPSCreg Name
STBCi026-A-4
Alternative name(s)
SFC840-03-03-PITX3-GFP
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 13th April 2021
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Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA5859482
CLO CLO_0103204
Cellosaurus CVCL_A4YP
Wikidata Q107116956

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493661

Ethics

Also have a look at the ethics information for the parental line STBCi026-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line STBCi026-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1

Characterisation

Differentiation Potency
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
TUJ-1
Yes
PITX3
Yes
Morphology
Dopaminergic neuronal differentiation

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
PITX3 (target)
Gene knock-in
10q24.32
GFP
10q24.32
PITX3-GFP tag inserted, single copy.
CRISPR-associated (CRISPR/Cas) System