STBCi026-A-3

SFC840-03-03 LRRK2 WT/R1441C H3

General

Cell Line

hPSCreg Name
STBCi026-A-3
Alternative name(s)
SFC840-03-03 LRRK2 WT/R1441C H3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th May 2022
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA5859481
Cellosaurus CVCL_A9A0
CLO CLO_0103008
Wikidata Q102114947

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493661

Ethics

Also have a look at the ethics information for the parental line STBCi026-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line STBCi026-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No gross karyotypic abnormalities observed, 6 small indels as in parent line
Passage number: 31
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Parkinson disease
Genetic modifications
LRRK2 (target)
Variant
12q12
NM_198578.4:c.4321C>T
NP_940980.4:p.Arg1441Cys
Heterozygous
Introduction of R1441C variant into wildtype iPSC line generated from individual with no known disease. CRISPR/Cas9-mediated double-strand break generated close locus (R1441C mutation) and homology-directed repair with donor sequence. See cell line CoA under 'Characterisation'.