PD57-7 MT8
ICGi052-B-1
General
Cell Line |
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| hPSCreg name | ICGi052-B-1 |
| Cite as: | ICGi052-B-1 |
| Alternative name(s) |
PD57-7 MT8
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 3rd December 2024 |
| User feedback | |
Provider |
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| Generator | Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG) |
| Owner | Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG) |
| Distributors | |
| Derivation country | Russia |
External Databases |
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| BioSamples | SAMEA117400231 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Family history | All female relatives are affected. |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
NCBI SRR accession: SAMN42050731, BioProject PRJNA563295 Detailed analysis of the clinical exome sequencing data of the patient's PBMCs has revealed a mutation in the MAPT gene (c.2013T>G, rs63750756). |
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMN42050731 |
Ethics
Also have a look at the ethics information for the parental line
ICGi052-B
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| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
ICGi052-B.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Gelatin | ||||||||||||||||||
| Feeder cells |
Mouse embryonic fibroblasts Cellfinder Ont Id: EFO_0004040 |
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| Passage method |
Enzymatically
TrypLE
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| O2 Concentration | 20 % | ||||||||||||||||||
| CO2 Concentration | 5 % | ||||||||||||||||||
| Medium |
Other medium:
Base medium: KnockOut DMEM
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| TRA 1-60 |
Yes |
Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| CD29 |
Yes |
| Actin, alpha 2, smooth muscle, aorta |
Yes |
Morphology
Immunofluorescent analysis for mesoderm marker alfa SMA (red signal), CD29 (green signal) in ICGi052-B-1 iPSC. DAPI (blue signal)
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Karyotyping and G-banding show ICGi052-B-1 iPSCs have a normal 46,XX karyotype at passage 10.
Passage number: 10
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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