ICGi021-A-1

K6-4fpCyto-13

General#

Cell Line

hPSCreg Name
ICGi021-A-1
Alternative name(s)
K6-4fpCyto-13
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th July 2021
Notes Healthy human iPSC clone with doxycycline-inducible expression of cytosolic Grx1-roGFP2
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Provider

Generator The Federal Research Center Institute of Cytology and Genetics The Siberian Branch of the Russian Academy of Sciences (ICG)
Owner The Federal Research Center Institute of Cytology and Genetics The Siberian Branch of the Russian Academy of Sciences (ICG)

External Databases

BioSamples SAMEA9000722
CLO CLO_0103411

General Information

* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Any
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
https://www.ncbi.nlm.nih.gov/sra/?term=SRR11413028
No disease associated mutation found

External Databases (Donor)

BioSamples SAMEA6983569

Ethics#

Also have a look at the ethics information for the parental line ICGi021-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line ICGi021-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells MEF cell
Cellfinder Ont Id: http://www.ebi.ac.uk/efo/EFO_0004040
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knock-out DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
GlutaMAX 2 mM
NEAA 0.1 mM
Penicillin-Streptomycin 100 U/ml
2-mercaptoethanol 0.1 mM
rhFGF basic 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CK-18
Yes
GATA6
Yes
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
aSMA
Yes
Collagen type I
Yes
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
Tubulin, beta 3
Yes
Neurofilament 200
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 9
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Healthy
Synonyms
  • Healthy
Genetic modifications
Genetic modifications not related to a disease
AAVS1 (target)
Transgene expression
19q13
Transgenes of glutathione redox sensor (cytosolic) cyto_Grx1-roGFP2 and tetracycline-transactivator (rtTA) in AAVS1 locus were detected by PCR.
CRISPR-associated (CRISPR/Cas) System
puro in.TIF
cyto Grx1-roGFP2 transgene detection
neo in.TIF
rtTA transgene detection