The cell line is not validated yet.
WAe009-A-1A
General
Cell Line |
|
| hPSCreg name | WAe009-A-1A |
| Cite as: | WAe009-A-1A (RRID:CVCL_D6K3) |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A (WA09, H9) MRIi001-A-1 (C6-AAVS1-iCasRx) ICGi021-A-1 (K6-4fpCyto-13) MRIi003-A-8 (HK-AAVS1-CAG-eGFP-homo) ICGi021-A-2 (K6-4fpCyto-16) ICGi021-A-3 (K6-4fpCyto-19) UCSFi001-A-12 (AICS-0036-006) MRIi003-A-7 (HK-AAVS1-iCasRx) UCSFi001-A-23 (AICS-0054-091) BIHi001-A-1 (iBCRT Cas9v1-3G-Kl.16) UCSFi001-A-36 (AICS-0036-028) CRMi003-A-3 (Proliving, GMNN-mScarletI Reporter) WAe009-A-1H (MYL3-KO) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-58 (COL4A5 heterozygote) WAe009-A-1P (IsoHD-30Q-NGN2) WAe009-A-59 (dHAND KO) WAe009-A-1Q (IsoHD-65Q-NGN2) WAe009-A-1R (IsoHD-81Q-NGN2) WAe009-A-1W (H9 EF1A-mCherry-noPuro 803_AAVS-iSHH #3) WAe009-A-60 (H9 iNK5A) |
| Last update | 15th October 2023 |
| User feedback | |
Provider |
|
| Generator |
Southern Medical University (SMUDH)
Contact:
Southern Medical University (SMUDH) |
| Owner | Southern Medical University (SMUDH) |
| Distributors | |
External Databases |
|
| BioSamples | SAMEA114492926 |
| Cellosaurus | CVCL_D6K3 |
| Wikidata | Q127384681 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
TrypLE
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|||||
| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
|
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