The cell line is not validated yet.
WAe009-A-3K
General
Cell Line |
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hPSCreg name | WAe009-A-3K |
Cite as: | WAe009-A-3K |
Cell line type | Human embryonic stem cell (hESC) |
Similar lines | No similar lines found. |
Last update | 16th September 2025 |
Notes | To obtain cell line contact: Claudia Doege; cad2114@cumc.columbia.edu or Carlos Tristan; carlos.tristan@nih.gov |
User feedback | |
Provider |
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Generator | National Institutes of Health - National Center for Advancing Translational Sciences (NCATS) |
Derivation country | United States |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | female |
Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Family history | NO |
Is the medical history available upon request? | NO |
Is clinical information available? | NO |
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
Is there an MTA available for the cell line? | Yes |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
The source cell information can be found in the parental cell line
WAe009-A.
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Culture Conditions
Surface coating | Vitronectin | |||||||||||||||
Feeder cells |
No |
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Passage method |
Enzyme-free cell dissociation
EDTA
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Medium |
Essential 8™
Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Unknown |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Unknown |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Unknown |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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NANOG |
Yes |
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SOX2 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XX
Passage number: 32
Karyotyping method:
Array CGH
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Other Genotyping (Cell Line) |
Genetic Modification
Genetic modifications not related to a disease |
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