WAe009-A-Z
General
Cell Line |
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| hPSCreg name | WAe009-A-Z |
| Cite as: | WAe009-A-Z |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A (WA09, H9) WAe009-A-1G (ISL1-KO) WAe009-A-59 (dHAND KO) WAe009-A-1H (MYL3-KO) WAe009-A-36 (JPH2-KO) WAe009-A-1E (TBX20-KO) WAe009-A-62 (KCNQ1 KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-72 (COL1A2 -/-) WAe009-A-1P (IsoHD-30Q-NGN2) WAe009-A-40 (H9 CBFA2T3-GLIS2, H9 ETO2-GLIS2) WAe009-A-1Q (IsoHD-65Q-NGN2) WAe009-A-1R (IsoHD-81Q-NGN2) WAe009-A-60 (H9 iNK5A) WAe009-A-81 (H9-MSX1-/- cell line) WAe009-A-77 (H9-GFP) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-99 (dCas9-p300 H9 21) |
| Last update | 1st November 2023 |
| User feedback | |
Provider |
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| Generator | ZhengZhou University- The first affiliated hospital(ZZU) (ZZUNEU) |
External Databases |
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| BioSamples | SAMEA114514495 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
hESC Derivation
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The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
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| SOX2 |
Yes |
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| OCT4 |
Yes |
Morphology pictures
Differentiation Potency
In vitro directed differentiation
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
naomal karyotype
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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