Generation of a TAB2 knockout hESC line (WAe009-A-Z) derived from H9 using CRISPR/Cas9
Summary
TGF-β-activated kinase 1 binding protein 2 (TAB2) is an intermediate protein that connects TNFR1 and other receptor signals to the TGF-β-activated kinase 1 (TAK1) signaling complex. TAB2 has been proved clinically relevant to congenital heart defects (CHD) and cardiomyopathy. In this study, we created a TAB2 knockout human embryonic stem cell line by CRISPR/Cas9 technology. The WAe009-A-Z cell line displayed stem cell morphology, pluripotency and normal karyotype, which could develop into three germ layers in vitro. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Sun W, Li X, Dong J, Zhou Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Feb;74:103284 |
| PubMed | 38118206 |
| DOI | 10.1016/j.scr.2023.103284 |