The cell line is not validated yet.
WAe009-A-3J
General
Cell Line |
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| hPSCreg name | WAe009-A-3J |
| Cite as: | WAe009-A-3J |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A (WA09, H9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-2D (TG.(ALK WT-GFP)) WAe009-A-2E (TG.(ALK F1174L-GFP)) WAe009-A-3L (H9-idCK-mC) WAe009-A-3M (H9-dCK-mC) WAe009-A-3N (H9-dCk) WAe009-A-58 (COL4A5 heterozygote) WAe009-A-1P (IsoHD-30Q-NGN2) WAe009-A-59 (dHAND KO) WAe009-A-1Q (IsoHD-65Q-NGN2) WAe009-A-1R (IsoHD-81Q-NGN2) WAe009-A-1W (H9 EF1A-mCherry-noPuro 803_AAVS-iSHH #3) WAe009-A-60 (H9 iNK5A) WAe009-A-1X (H9 ALK CRISPR F1174L) WAe009-A-1H (MYL3-KO) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) |
| Last update | 16th September 2025 |
| Notes | To obtain cell line contact: Claudia Doege; cad2114@cumc.columbia.edu or Carlos Tristan; carlos.tristan@nih.gov |
| User feedback | |
Provider |
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| Generator | National Institutes of Health - National Center for Advancing Translational Sciences (NCATS) |
| Derivation country | United States |
External Databases |
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General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Additional restrictions:
MTA required |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
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The source cell information can be found in the parental cell line
WAe009-A.
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Culture Conditions
| Surface coating | Vitronectin | |||||||||||||||
| Feeder cells |
No |
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| Passage method |
Enzyme-free cell dissociation
EDTA
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| Medium |
Essential 8™
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Unknown |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Unknown |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Unknown |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XX
Passage number: 32
Karyotyping method:
Array CGH
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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