WAe009-A-3N

H9-dCk

The cell line is not validated yet.

General

Cell Line

hPSCreg name WAe009-A-3N
Cite as:
WAe009-A-3N
Alternative name(s)
H9-dCk
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 12th December 2025
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Provider

Generator University of Texas Southwestern Medical Center (UTSW)

External Databases

BioSamples SAMEA120809657

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
TBXT
Yes
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Marker Expressed
TNNT2
Yes
MYH6
Yes
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
DCX
Yes
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
CLYBL locus
TALENS were used to insert CAG-dCas9-KRAB into clybl locus. EF1-mCherry cassette used to select clones with integration was clipped out using Cre recombinase.
TALEN