WAe009-A-3L

H9-idCK-mC

The cell line is not validated yet.

General

Cell Line

hPSCreg name WAe009-A-3L
Cite as:
WAe009-A-3L
Alternative name(s)
H9-idCK-mC
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 25th September 2025
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Provider

Generator University of Texas Southwestern Medical Center (UTSW)
Owner University of Texas Southwestern Medical Center (UTSW)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA120247127

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Cardiomyocyte
Ont Id: NCIT_C13002

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
CLYBL locus
TRE-dCas9-KRAB inserted into clybl locus using talens
TALEN
Transgene expression
rosa26 locus
rtTA was inserted into Rosa26 locus using specific sgRNA and Cas9.
CRISPR-associated (CRISPR/Cas) System