H9 EF1A-mCherry-noPuro 803_AAVS-iSHH #3
WAe009-A-1W
General
Cell Line |
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| hPSCreg name | WAe009-A-1W |
| Cite as: | WAe009-A-1W |
| Alternative name(s) |
H9 EF1A-mCherry-noPuro 803_AAVS-iSHH #3
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A (WA09, H9) WAe009-A-99 (dCas9-p300 H9 21) WAe009-A-1V (H9-AAVS1-Teton-KrasG12D) WAe009-A-62 (KCNQ1 KO) WAe009-A-1G (ISL1-KO) WAe009-A-90 (H9_LCCS1) WAe009-A-72 (COL1A2 -/-) WAe009-A-77 (H9-GFP) WAe009-A-1H (MYL3-KO) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) |
| Last update | 25th March 2024 |
| User feedback | |
Provider |
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| Generator | Memorial Sloan Kettering Cancer Center (MSK) |
External Databases |
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| BioSamples | SAMEA115433692 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
hESC Derivation
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The source cell information can be found in the parental cell line
WAe009-A.
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Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
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| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Differentiation Potency
In vitro directed differentiation
Protocol or reference
Cederquist et al, 2019 Nat Biotech. .pdf
In the study of Cederquist et al, 2019 Nat Biotech, the authors used this inducible Sonic Hedgehog (SHH) hESC line in developing forebrain organoids that enables ordered self-organization along dorso-ventral and anteroposterior positional axes. The SHH-patterned forebrain organoids establish major forebrain subdivisions that are positioned with in vivo-like topography. In the figure 1, the data present the establishment of the iSHH line, and the dorso-ventral and antero-posterior developmental axes in SHH differentiated forebrain organoids.
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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