JPH2-KO
WAe009-A-36
General#
Cell Line |
|
hPSCreg Name | WAe009-A-36 |
Alternative name(s) |
JPH2-KO
|
Cell line type | Human embryonic stem cell (hESC) |
Last update | 10th August 2020 |
User feedback | |
Provider |
|
Generator |
Anzhen Hospital (ANZHEN)
Contact:
Anzhen Hospital (ANZHEN) |
Owner | Anzhen Hospital (ANZHEN) |
Distributors | |
Derivation country | China |
External Databases |
|
CLO | CLO_0102925 |
BioSamples | SAMEA7258631 |
Cellosaurus | CVCL_ZX63 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: allowed
Commercial: not allowed
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Subclone of |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
|
Family history | NO |
Is the medical history available upon request? | NO |
Is clinical information available? | NO |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Yes
Karyotyping method:
G-Banding
|
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
|
BioSamples | SAMEA7768918 |
Ethics#
Also have a look at the ethics information for the parental line
WAe009-A
.
Is there an MTA available for the cell line? | No |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation#
Date of derivation | 1998-01-01 |
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
PGD Embryo? |
No |
Cell isolation | Immunosurgery |
Cell seeding | Isolated ICM |
Derived under xeno-free conditions? |
No |
Derivation under GMP? |
No |
Available as clinical grade? |
Yes |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Certificate of Analysis |
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Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
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