Generation of a TBX20-knockout human embryonic stem line by CRISPR/Cas9 system


The TBX20 gene plays a crucial role in embryonic development and has been involved in various diseases, such as heart defects, intellectual disability, and cancer. Herein, we have established a TBX20-knockout human embryonic stem cell line (WAe009-A-84) that maintains stem cell-like features, pluripotency, a normal karyotype, and the ability to differentiate into all three germ layers in vivo. This cell line will be a valuable resource for exploring TBX20's role in human development and could have significant implications for regenerative medicine and disease modeling. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Wu X, Wang X
Journal Stem cell research
Publication Date 2023 Jun;69:103082
PubMed 36963213
DOI 10.1016/j.scr.2023.103082

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