Generation of a Junctophilin-2 homozygous knockout human embryonic stem cell line (WAe009-A-36) by an episomal vector-based CRISPR/Cas9 system

Summary

Mutations in Junctophilin-2(JPH2) gene is the cause of hypertrophic cardiomyopathy (HCM) and leading inherited cause of left ventricular hypertrophy and myofilaments disarray. JPH2 protein, a member of the Junctophilin family, is mainly expressed in heart and plays an important role in E-C coupling. We have generated a homozygous JPH2 knockout (JPH2-KO) human embryonic stem cell (hESC) line using an episomal vector-based CRISPR/Cas9 system. This JPH2-KO hESC line maintained stem cell like morphology, pluripotency, normal karyotype and could differentiate into all three germ layers in vivo. Copyright © 2020. Published by Elsevier B.V.

Authors Wu F, Li X, Bai R, Li Y, Gao J, Lan F
Journal Stem cell research
Publication Date 2020 Aug 3;48:101930
PubMed 32777767
DOI 10.1016/j.scr.2020.101930

Research Projects

Cell Lines