Generation of a TPM1 homozygous knockout embryonic stem cell line by CRISPR/Cas9 editing


Alpha-Tropomyosin (TPM1) plays a crucial role in actin regulation and stability and contributes fundamental functions to heart development: without TPM1 expressing, mice embryos will die early in embryogenesis. To further identify the role of TPM1 in human cardiac development, here we generated a homozygous TPM1 knockout (TPM1-/-) human embryonic stem cell (hESC) line using CRISPR/Cas9-based genome editing system. The generated TPM1-/- hESC line maintained normal karyotype, highly expressed pluripotency markers and was able to differentiate into all three germ layers in vivo. This cell line provides a powerful tool to investigate the role of TPM1 in heart development in future. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Ma S, Xu Q, Bai R, Dong T, Peng Z, Liu X
Journal Stem cell research
Publication Date 2021 Aug;55:102470
PubMed 34352617
DOI 10.1016/j.scr.2021.102470

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