WAe009-A-1G

ISL1-KO

General

Cell Line

hPSCreg name WAe009-A-1G
Cite as:
WAe009-A-1G (RRID:CVCL_D6PC)
Alternative name(s)
ISL1-KO
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
WAe009-A-59
(dHAND KO)
WAe009-A-1H
(MYL3-KO)
WAe009-A-36
(JPH2-KO)
WAe009-A-1E
(TBX20-KO)
WAe009-A-62
(KCNQ1 KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-72
(COL1A2 -/-)
WAe009-A-1P
(IsoHD-30Q-NGN2)
WAe009-A-40
(H9 CBFA2T3-GLIS2, H9 ETO2-GLIS2)
WAe009-A-1Q
(IsoHD-65Q-NGN2)
WAe009-A-1R
(IsoHD-81Q-NGN2)
WAe009-A-60
(H9 iNK5A)
WAe009-A-81
(H9-MSX1-/- cell line)
WAe009-A-77
(H9-GFP)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-99
(dCas9-p300 H9 21)
Last update 15th October 2023
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Provider

Generator Southwest Medical University (SWMU)
Owner Southwest Medical University (SWMU)
Distributors
Derivation country China

External Databases

BioSamples SAMEA114498216
Cellosaurus CVCL_D6PC

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution The cell is available from the corresponding author upon reasonable request.

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Congenital Heart Disease
Genetic modifications
ISL1 (target)
Gene knock-out
5q11.1
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease
ISL1 (target)
Gene knock-out
5q11.1
CRISPR-associated (CRISPR/Cas) System