The cell line is not validated yet.
WAe009-A-3I
General
Cell Line |
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| hPSCreg name | WAe009-A-3I |
| Cite as: | WAe009-A-3I |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 16th September 2025 |
| Notes | To obtain cell line contact: Claudia Doege; cad2114@cumc.columbia.edu or Carlos Tristan; carlos.tristan@nih.gov |
| User feedback | |
Provider |
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| Generator | National Institutes of Health - National Center for Advancing Translational Sciences (NCATS) |
| Derivation country | United States |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Additional restrictions:
MTA required |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
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The source cell information can be found in the parental cell line
WAe009-A.
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Culture Conditions
| Surface coating | Vitronectin | |||||||||||||||
| Feeder cells |
No |
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| Passage method |
Enzyme-free cell dissociation
EDTA
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| Medium |
Essential 8™
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Unknown |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Unknown |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Unknown |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
| Genetic modifications not related to a disease |
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