H9-NRL-GP, WA09 NRL+/eGFP

General

Cell Line

hPSCreg name WAe009-A-R
Cite as:
WAe009-A-R (RRID:CVCL_B5ZI)
Alternative name(s)
H9-NRL-GP, WA09 NRL+/eGFP
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
WAe009-A-77
(H9-GFP)
WAe009-A-1H
(MYL3-KO)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-36
(JPH2-KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-1E
(TBX20-KO)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-99
(dCas9-p300 H9 21)
WAe009-A-1V
(H9-AAVS1-Teton-KrasG12D)
WAe009-A-62
(KCNQ1 KO)
WAe009-A-1G
(ISL1-KO)
WAe009-A-90
(H9_LCCS1)
WAe009-A-72
(COL1A2 -/-)
WAe009-A-58
(COL4A5 heterozygote)
Last update 30th June 2023
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Provider

Generator WiCell Research Institute (WA)
Owner University of Wisconsin (WISC)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA113967375
Cellosaurus CVCL_B5ZI
Wikidata Q112041918

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:

MTA required

Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Cultrex
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Unknown

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
Created human rod reporter line using CRISPR/Cas9 genome editing to replace one allele of the Neural Retina Leucine zipper (NRL) gene with an eGFP transgene in the WA09 human embryonic stem cell (hESC) line.
CRISPR-associated (CRISPR/Cas) System