H9_RB1ex3_C7
WAe009-A-12
General
Cell Line |
|
| hPSCreg name | WAe009-A-12 |
| Cite as: | WAe009-A-12 (RRID:CVCL_UG28) |
| Alternative name(s) |
H9_RB1ex3_C7
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A (WA09, H9) RUCDRi002-A-63 (LVIP05-RB1-CS1) WAe001-A-31 (H1_RB1ex1_D6) WAe001-A-10 (H1_RB1ex3_G3) WAe001-A-11 (H1_RB1ex3_G4) WAe009-A-40 (H9 CBFA2T3-GLIS2, H9 ETO2-GLIS2) WAe009-A-60 (H9 iNK5A) WAe009-A-1P (IsoHD-30Q-NGN2) WAe009-A-1Q (IsoHD-65Q-NGN2) WAe009-A-1R (IsoHD-81Q-NGN2) WAe009-A-1H (MYL3-KO) WAe009-A-36 (JPH2-KO) WAe009-A-1E (TBX20-KO) WAe009-A-62 (KCNQ1 KO) WAe009-A-1G (ISL1-KO) WAe009-A-72 (COL1A2 -/-) WAe009-A-59 (dHAND KO) WAe009-A-81 (H9-MSX1-/- cell line) WAe009-A-77 (H9-GFP) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) |
| Last update | 26th June 2019 |
| User feedback | |
Provider |
|
| Generator |
Universität Duisburg-Essen (UDE)
Contact:
Universitätsklinikum Essen |
| Owner | Universitätsklinikum Essen |
| Distributors | |
| Derivation country | Germany |
External Databases |
|
| Cellosaurus | CVCL_UG28 |
| BioSamples | SAMEA5912511 |
| Wikidata | Q98134724 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: StemMACS iPS-Brew XF, human
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XX
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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