WAe001-A-11

H1_RB1ex3_G4

General#

Cell Line

hPSCreg Name WAe001-A-11
Alternative name(s)
H1_RB1ex3_G4
Cell line type Human embryonic stem cell (hESC)
Last update 1st August 2018
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Provider

Generator Universität Duisburg-Essen (UDE)
Owner Universitätsklinikum Essen
Distributors
Derivation country Germany

External Databases

Cellosaurus CVCL_VE16
BioSamples SAMEA7767418

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7767418

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2016-01-13
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Retinoblastoma
Genetic modifications
RB1 (target)
Gene knock-out
13q14
insertion of two nucleotides (AA) / deletion of seven nucleotides in exon 3 of RB1 gene
CRISPR-associated (CRISPR/Cas) System