H1_RB1ex3_G4
WAe001-A-11
General#
Cell Line |
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| hPSCreg Name | WAe001-A-11 |
| Alternative name(s) |
H1_RB1ex3_G4
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 1st August 2018 |
| User feedback | |
Provider |
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| Generator | Universität Duisburg-Essen (UDE) |
| Owner | Universitätsklinikum Essen |
| Distributors | |
| Derivation country | Germany |
External Databases |
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| Cellosaurus | CVCL_VE16 |
| BioSamples | SAMEA7767418 |
General Information |
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| Publications | |
| Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7767418 |
Ethics#
We are currently revising this section.hESC Derivation#
| Date of derivation | 2016-01-13 |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
Culture Conditions#
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification#
| Disease/phenotype related modifications |
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