H1_RB1ex3_G4
WAe001-A-11
General#
Cell Line |
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hPSCreg Name | WAe001-A-11 |
Alternative name(s) |
H1_RB1ex3_G4
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 1st August 2018 |
User feedback | |
Provider |
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Generator | Universität Duisburg-Essen (UDE) |
Owner | Universitätsklinikum Essen |
Distributors | |
Derivation country | Germany |
External Databases |
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Cellosaurus | CVCL_VE16 |
BioSamples | SAMEA7767418 |
General Information |
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Publications | |
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Subclone of |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA7767418 |
Ethics#
We are currently revising this section.hESC Derivation#
Date of derivation | 2016-01-13 |
Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
PGD Embryo? |
No |
Culture Conditions#
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SOX2 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
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