WAe001-A-A

General

Cell Line
hPSCreg name	WAe001-A-A
Cite as:	WAe001-A-A (RRID:CVCL_C9K8)
Alternative name(s)	H1X-10/H1.2-20
Cell line type	Human embryonic stem cell (hESC)
Similar lines	WAe001-A-B (H1X-59/H1.2-6) WAe001-A-K (H1.0-29/H1.2-31) WAe001-A-L (H1.0-102/H1.2-6) WAe001-A-S (H1.1-17/H1.2-11) WAe001-A-T (H1.1-39/H1.2-9) WAe001-A-87 (H1.213, H1.2-13) WAe001-A-88 (H1.2-124, H1.2124) WAe001-A (WA01, H1) WAe001-A-2B (H1-NSD2-/-) WAe001-A-2H WAe001-A-C (H1X-10/H1.3-62) WAe001-A-3L WAe001-A-D (H1X-59/H1.3-68) WAe001-A-3M WAe001-A-E (H1X-10/H1.4-4) WAe001-A-3N WAe001-A-F (H1X-59/H1.4-5) WAe001-A-G (H1X-10/H1.5-1) WAe001-A-2J WAe001-A-1N (H1-FIS1-KO, WA01-FIS1-KO) WAe001-A-H (H1X-59/H1.5-33) WAe001-A-1O (WA01-MYB-KO, H1-MYB-KO) WAe001-A-I (H1.0-29/H1.1-13) WAe001-A-J (H1.0-102/H1.1-34) WAe001-A-2E WAe001-A-2K WAe001-A-1 (H1.1, WA01.1) WAe001-A-M (H1.0-29/H1.3-22) WAe001-A-N (H1.0-102/H1.3-90) WAe001-A-3A WAe001-A-3O WAe001-A-O (H1.0-29/H1.4-14) WAe001-A-P (H1.0-102/H1.4-19) WAe001-A-Q (H1.0-29/H1.5-6) WAe001-A-73 (H1-GATAD2B-KO-79#) WAe001-A-R (H1.0-102/H1.5-44) WAe001-A-81 WAe001-A-74 (H1-GATAD2B-KO-81#) WAe001-A-U (H1.1-17/H1.4-18) WAe001-A-V (H1.1-39/H1.4-17) WAe001-A-W (H1.1-17/H1.5-14) WAe001-A-X (H1.1-39/H1.5-26) WAe001-A-Y (H1.2-13/H1.3-35) WAe001-A-2F WAe001-A-Z (H1.2-124/H1.3-23) WAe001-A-82 (H1X-59, H1X59) WAe001-A-2C (SST-P2A-mCherry) WAe001-A-3S (H1-FCGR3A-EGFP) WAe001-A-1A (H1.2-13/H1.4-69) WAe001-A-1B (H1.2-124/H1.4-84)
Last update	6th December 2022
Notes	Linker histones H1X/H1.2 double knockout. The H1.2 linker histone was knocked out from WAe001-A-78 (H1X single knockout) subclone.
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Provider
Generator	King Abdullah University of Science and Technology (KAUST) Contact: King Abdullah University of Science and Technology (KAUST)
Owner	King Abdullah University of Science and Technology (KAUST)
Distributors	King Abdullah University of Science and Technology (KAUST)
Derivation country	Saudi Arabia
External Databases
BioSamples	SAMEA112192699
Cellosaurus	CVCL_C9K8
Wikidata	Q123033829
General Information
Publications	Hossini AM et al. PI3K/AKT Signaling Pathway Is Essential for Survival of Induced Pluripotent Stem Cells. PloS one. 2016;11(5):e0154770. Xu C et al. Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling. Nature communications. 2016 Nov 4;7:13287. Xu Y et al. Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9. Stem cell research. 2017 Jul;22:29-32. Liu Y et al. Generation of two MEN1 knockout lines from a human embryonic stem cell line. Stem cell research. 2017 Oct;24:169-173. Liu Y et al. Generation of three miR-122 knockout lines from a human embryonic stem cell line. Stem cell research. 2017 Oct;24:164-168. Yuan F et al. Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9. Stem cell research. 2017 Oct;24:89-93. Yuan F et al. Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. Stem cell research. 2018 Jan;26:67-71. Böiers C et al. A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1. Developmental cell. 2018 Feb 5;44(3):362-377.e7. Wu F et al. Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. Stem cell research. 2018 Mar;27:5-9. Xu G et al. Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing. Stem cell research. 2018 Mar;27:38-41. Liu Y et al. Generation of WAe001-A-15, a human embryonic stem cell line with miR-122 doxycycline-inducible expression. Stem cell research. 2018 May;29:84-87. Xu A et al. Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing. Stem cell research. 2018 May;29:215-219. Guhr A et al. Recent Trends in Research with Human Pluripotent Stem Cells: Impact of Research and Use of Cell Lines in Experimental Research and Clinical Trials. Stem cell reports. 2018 Aug 14;11(2):485-496. Wei R et al. Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing. Stem cell research. 2018 Oct;32:139-144. Hariharan K et al. Parallel generation of easily selectable multiple nephronal cell types from human pluripotent stem cells. Cellular and molecular life sciences : CMLS. 2019 Jan;76(1):179-192. Xue Y et al. Establishment of an ectodermal dysplasia related gene EDA Knockout human embryonic stem cell line (WAe001-A-22) by CRISPR-Cas9 technology. Stem cell research. 2019 Jan;34:101379. Xiang Y et al. hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids. Cell stem cell. 2019 Mar 7;24(3):487-497.e7. Uniyal AP et al. An overview of designing and selection of sgRNAs for precise genome editing by the CRISPR-Cas9 system in plants. 3 Biotech. 2019 Jun;9(6):223. Wen MH et al. Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1. Stem cell research. 2019 Dec;41:101631. Ast J et al. Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. Nature communications. 2020 Jan 24;11(1):467. Alexeeva V et al. A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing. Stem cell research. 2020 May;45:101837. Fan C et al. TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46. Stem cell research. 2020 Jul;46:101854. Kobold S et al. A Manually Curated Database on Clinical Studies Involving Cell Products Derived from Human Pluripotent Stem Cells. Stem cell reports. 2020 Aug 11;15(2):546-555. Wang L et al. Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly. Nature communications. 2020 Aug 12;11(1):4038. Ast J et al. Author Correction: Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. Nature communications. 2020 Oct 9;11(1):5160. Minn KT et al. High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures. eLife. 2020 Nov 18;9. Sun Y et al. Applications of piggyBac Transposons for Genome Manipulation in Stem Cells. Stem cells international. 2021;2021:3829286. Quan Y et al. Generation of a human embryonic stem cell (WAe001-A-47) with hVGLL4 doxycyclin-inducible expression by the PiggyBac transposon system. Stem cell research. 2021 Jan;50:102142. Crawford LB et al. CD34(+) Hematopoietic Progenitor Cell Subsets Exhibit Differential Ability To Maintain Human Cytomegalovirus Latency and Persistence. Journal of virology. 2021 Jan 13;95(3). Wang L et al. A Human 3D neural assembloid model for SARS-CoV-2 infection. bioRxiv : the preprint server for biology. 2021 Feb 9;. Yang Z et al. Generation of a DKK1 homozygous knockout human embryonic stem cell line WAe001-A-21 using CRISPR/Cas9. Stem cell research. 2021 Mar;51:102161. Huang Y et al. Generation of an EFNB2-2A-mCherry reporter human embryonic stem cell line using CRISPR/Cas9-mediated site-specific homologous recombination. Stem cell research. 2021 Apr;52:102241. Paes BCMF et al. Generation of hematopoietic stem/progenitor cells with sickle cell mutation from induced pluripotent stem cell in serum-free system. Hematology, transfusion and cell therapy. 2021 Apr-Jun;43(2):156-164. Wu F et al. Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein. Stem cell research. 2021 May;53:102197. Xiao X et al. Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system. Stem cell research. 2021 May;53:102309. Richardson SE et al. In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture. STAR protocols. 2021 Jun 18;2(2):100420. Getachew A et al. Generation of a TLR2 homozygous knockout human embryonic stem cell line WAe001-A-64 using CRISPR/Cas9 editing. Stem cell research. 2021 Jul;54:102401. Huang PS et al. Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1. Stem cell research. 2021 Jul;54:102415. Quan Y et al. Generation of human embryonic stem cell lines (WAe001-A-67,WAe001-A-68) with TEAD1 and TEAD4 expression by the PiggyBac transposon system. Stem cell research. 2021 Jul;54:102408. Wang L et al. A human three-dimensional neural-perivascular 'assembloid' promotes astrocytic development and enables modeling of SARS-CoV-2 neuropathology. Nature medicine. 2021 Sep;27(9):1600-1606. Ngo TTT et al. Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system. Cell proliferation. 2022 Mar;55(3):e13190. Varga BV et al. Signal requirement for cortical potential of transplantable human neuroepithelial stem cells. Nature communications. 2022 May 23;13(1):2844. Astro V et al. Fine-tuned KDM1A alternative splicing regulates human cardiomyogenesis through an enzymatic-independent mechanism. iScience. 2022 Jul 15;25(7):104665. Chen YH et al. Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1. Stem cell research. 2022 Aug;63:102845. Miguel-Escalada I et al. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster. Developmental cell. 2022 Aug 22;57(16):1922-1936.e9. Hovland AS et al. Pluripotency factors are repurposed to shape the epigenomic landscape of neural crest cells. Developmental cell. 2022 Oct 10;57(19):2257-2272.e5. Prochazka L et al. Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells. Molecular systems biology. 2022 Nov;18(11):e10886. de Thonel A et al. CBP-HSF2 structural and functional interplay in Rubinstein-Taybi neurodevelopmental disorder. Nature communications. 2022 Nov 16;13(1):7002. Astro V et al. Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media. STAR protocols. 2023 Apr 13;4(2):102252. Overeem AW et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay. Cell reports methods. 2023 Jun 26;3(6):100488. Asmar AJ et al. A ubiquitin-based effector-to-inhibitor switch coordinates early brain, craniofacial, and skin development. Nature communications. 2023 Jul 26;14(1):4499. Han CZ et al. Human microglia maturation is underpinned by specific gene regulatory networks. Immunity. 2023 Sep 12;56(9):2152-2171.e13. Cheng R et al. A Combinatorial Regulatory Platform Determines Expression of RNA Polymerase III Subunit RPC7α (POLR3G) in Cancer. Cancers. 2023 Oct 15;15(20). Harper TC et al. GATA1 deletion in human pluripotent stem cells increases differentiation yield and maturity of neutrophils. iScience. 2023 Oct 20;26(10):107804. Dirck A et al. HCMV UL8 interaction with β-catenin and DVL2 regulates viral reactivation in CD34(+) hematopoietic progenitor cells. Journal of virology. 2023 Oct 31;97(10):e0124123. Reumann D et al. In vitro modeling of the human dopaminergic system using spatially arranged ventral midbrain-striatum-cortex assembloids. Nature methods. 2023 Dec;20(12):2034-2047. Shen M et al. Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development. Neuron. 2023 Dec 20;111(24):3988-4005.e11. Vandenbempt V et al. HAMSAB diet ameliorates dysfunctional signaling in pancreatic islets in autoimmune diabetes. iScience. 2024 Jan 19;27(1):108694. Pavon N et al. Patterning ganglionic eminences in developing human brain organoids using a morphogen-gradient-inducing device. Cell reports methods. 2024 Jan 22;4(1):100689. Zhang T et al. Generation of SST-P2A-mCherry reporter human embryonic stem cell line using the CRISPR/Cas9 system (WAe001-A-2C). Stem cell research. 2024 Jun;77:103397. Stanojević D et al. Rockfish: A transformer-based model for accurate 5-methylcytosine prediction from nanopore sequencing. Nature communications. 2024 Jul 3;15(1):5580. Wilhelmsen I et al. The effects of TGF-β-induced activation and starvation of vitamin A and palmitic acid on human stem cell-derived hepatic stellate cells. Stem cell research & therapy. 2024 Jul 23;15(1):223. Chen J et al. Generation of FOXJ1-EGFP knock-in reporter human embryonic stem cell line, WAe001-A-2D, using CRISPR/Cas9-based gene targeting. Stem cell research. 2024 Aug;78:103445. Shan Y et al. METTL3/METTL14 maintain human nucleoli integrity by mediating SUV39H1/H2 degradation. Nature communications. 2024 Aug 21;15(1):7186. Jiang S et al. Generation of ASCL1-mCherry knock-in reporter in human embryonic stem cell line, WAe001-A-2E, using CRISPR/Cas9-based gene targeting. Stem cell research. 2024 Oct;80:103500. Diggins NL et al. Viral microRNA regulation of Akt is necessary for reactivation of Human Cytomegalovirus from latency in CD34+ hematopoietic progenitor cells and humanized mice. PLoS pathogens. 2024 Dec;20(12):e1012285. Leavens KF et al. Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background. Stem cell research. 2024 Dec;81:103559. Sun H et al. Generation of SFTPC-mCherry knock-in reporter human embryonic stem cell line, WAe001-A-2H, using CRISPR/Cas9-based gene targeting. Stem cell research. 2024 Dec;81:103597. Amirola-Martinez M et al. Aspects of zone-like identity and holotomographic tracking of human stem cell-derived liver sinusoidal endothelial cells. Frontiers in cell and developmental biology. 2025;13:1528991. Chidiac R et al. Selective activation of FZD2 and FZD7 reveals non-redundant function during mesoderm differentiation. Stem cell reports. 2025 Feb 11;20(2):102391. Pavlinek Adam et al. Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines. . 2025-03-24. Qi X et al. Generation of CNPY3 knock out cell line in the H1 (WA01) hESC background. Stem cell research. 2025 Apr;84:103676. Abraham E et al. A retinoic acid:YAP1 signaling axis controls atrial lineage commitment. Cell reports. 2025 May 27;44(5):115687. Tidball AM et al. Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Cell reports. 2025 Jun 24;44(6):115842. Zhao G et al. The role of ASS1 in mouse embryonic stem cell differentiation into mesendoderm lineages. Stem cell research & therapy. 2025 Sep 1;16(1):479. Richards A et al. A human blood-brain barrier model reveals pericytes as critical regulators of viral neuroinvasion. iScience. 2026 Jan 16;29(1):114443. Tardaguila M et al. Integrating natural and engineered genetic variations to decode regulatory influence on blood traits. Cell reports. 2026 Feb 24;45(2):116937. Yao F et al. Generation of a SV2A knockout human embryonic stem cell line by CRISPR/Cas9 system. Stem cell research. 2026 Mar;91:103924.
* Is the cell line readily obtainable for third parties?	No
Subclone of	WAe001-A

Donor Information

General Donor Information

Sex

male

Ethnicity

N/A

Phenotype and Disease related information (Donor)

Diseases

No disease was diagnosed.

Disease associated phenotypes

no phenotypes

Family history

NO

Is the medical history available upon request?

NO

Is clinical information available?

NO

Karyotyping (Donor)

Has the donor karyotype been analysed?

Yes

46, XY

WA01-WT.pdf

Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?

No

Donor Relations

Other cell lines of this donor

All cell lines of this donor's relatives

Has brother:

External Databases (Donor)

BioSamples

SAMEA7767418

Ethics

Also have a look at the ethics information for the parental line WAe001-A .

Is there an MTA available for the cell line?	No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above?	No

hESC Derivation

The source cell information can be found in the parental cell line WAe001-A.

Culture Conditions

Surface coating	Laminin
Feeder cells	No
Passage method	Enzymatically Accutase
CO2 Concentration	5 %
Medium	Essential 8™ Culture_conditions_of_WAe001_H1_knock-outs.pdf
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
SOX2	Yes			–
NANOG	Yes			–
OCT4	Yes			–
OCT3	Yes

Morphology

Morphology pictures

morphology H1X-10-H1.2-20.pdf

Pluripotent morphology

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro directed differentiation

Marker	Expressed
FOXA2	Yes
SOX17	Yes

Morphology

H1X-10H1.2-20 Endoderm differentiation.pdf

endodermal differentiation

Protocol or reference

differentiation protocol.pdf

direct differentiation protocol

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro directed differentiation

Marker	Expressed
Brachyury	Yes
NCAM	Yes

Morphology

H1X-10H1.2-20 Mesoderm differentiation.pdf

mesoderal differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro directed differentiation

Marker	Expressed
Nestin	Yes
SOX1	Yes
CD44	Yes

Morphology

H1X-10H1.2-20 Ectoderm differentiation.pdf

ectodermal differentiation

Microbiology / Virus Screening
Mycoplasma	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XY Passage number: +12 Karyotyping method: G-Banding
Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease

H1.2 (target)

Type of modification

Gene knock-out

Chromosome location

Chromosome 6: 26,055,740-26,056,470

Free text

The knockout was confirmed by Sanger sequencing, RT-qPCR, and Western blot to prove the absence of the sequence/protein. This cell line (H1.2-20 KO) was derived from the already knocked-out H1X-10 cell line (WAe001-A-78).

Delivery method

CRISPR-associated (CRISPR/Cas) System

Available documents

H1.X-10 genotyping.pdf
First knock-out confirmation H1X-10

H1X-10H1.2-20 genotyping.pdf
Second knock-out confirmation H1.2-20

H1X-10/H1.2-20

General

Cell Line

Provider

External Databases

General Information

Donor Information

General Donor Information

Phenotype and Disease related information (Donor)

Karyotyping (Donor)

Other Genotyping (Donor)

Donor Relations

External Databases (Donor)

Ethics

hESC Derivation

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells

Differentiation Potency

Microbiology / Virus Screening

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

H1.2 (target)