Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background
Summary
Over the past decade, the use of human stem cell-derived β cells (SC-β cells) to model pancreatic β cell development, function and disease has become increasingly common. Though protocols are rapidly improving, current directed differentiation strategies do not yield a pure population of insulin-positive SC-β cells in vitro. Therefore, it is experimentally advantageous to have reporter lines that allow for live sorting of insulin-positive populations. To aid in these studies, we have knocked mNeonGreen fluorescent protein into the endogenous insulin locus of the commonly used H1 (WA01) human embryonic stem cell line. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Leavens KF, Osorio-Quintero C, Yeuteuh EA, Perez-Profeta FT, Dattoli AA, Cardenas-Diaz FL, French DL, Gadue P |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Dec;81:103559 |
| PubMed | 39276530 |
| PubMed Central | PMC11719772 |
| DOI | 10.1016/j.scr.2024.103559 |