H1 INS-PPImNG, CHOPe004-A-2I
WAe001-A-2I
General
Cell Line |
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| hPSCreg name | WAe001-A-2I |
| Cite as: | WAe001-A-2I |
| Alternative name(s) |
H1 INS-PPImNG, CHOPe004-A-2I
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 9th August 2024 |
| Notes | heterozygous knock-in of mNeonGreen fluorescent protein into the endogenous insulin locus of the H1 (WA01) human embryonic stem cell line |
| User feedback | |
Provider |
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| Generator | The Children's Hospital of Philadelphia (CHOP) |
External Databases |
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| BioSamples | SAMEA115909935 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
Must have MTA for WAe001-A |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has brother:
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External Databases (Donor) |
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| BioSamples | SAMEA7767418 |
Ethics
Also have a look at the ethics information for the parental line
WAe001-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
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The source cell information can be found in the parental cell line
WAe001-A.
|
Culture Conditions
| Surface coating | Gelatin |
| Feeder cells |
MEFs |
| Passage method |
Enzymatically
TrypLE
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement Serum concentration: 20 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY
Passage number: 14
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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