GZLe001-C
General
Cell Line |
|
| hPSCreg name | GZLe001-C |
| Cite as: | GZLe001-C |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe001-A-A (H1X-10/H1.2-20) WAe001-A-2B (H1-NSD2-/-) WAe001-A-B (H1X-59/H1.2-6) WAe001-A-C (H1X-10/H1.3-62) WAe001-A-D (H1X-59/H1.3-68) WAe001-A-E (H1X-10/H1.4-4) WAe001-A-F (H1X-59/H1.4-5) WAe001-A-G (H1X-10/H1.5-1) WAe001-A-1N (H1-FIS1-KO, WA01-FIS1-KO) WAe001-A-H (H1X-59/H1.5-33) WAe001-A-1O (WA01-MYB-KO, H1-MYB-KO) WAe001-A-I (H1.0-29/H1.1-13) WAe001-A-J (H1.0-102/H1.1-34) WAe001-A-K (H1.0-29/H1.2-31) WAe001-A-1 (H1.1, WA01.1) WAe001-A-L (H1.0-102/H1.2-6) WAe001-A-M (H1.0-29/H1.3-22) WAe001-A-N (H1.0-102/H1.3-90) WAe001-A-O (H1.0-29/H1.4-14) WAe001-A-P (H1.0-102/H1.4-19) WAe001-A-Q (H1.0-29/H1.5-6) WAe001-A-73 (H1-GATAD2B-KO-79#) WAe001-A-R (H1.0-102/H1.5-44) WAe001-A-74 (H1-GATAD2B-KO-81#) WAe001-A-S (H1.1-17/H1.2-11) WAe001-A-T (H1.1-39/H1.2-9) WAe001-A-U (H1.1-17/H1.4-18) WAe001-A-V (H1.1-39/H1.4-17) WAe001-A-W (H1.1-17/H1.5-14) WAe001-A-X (H1.1-39/H1.5-26) WAe001-A-Y (H1.2-13/H1.3-35) WAe001-A-Z (H1.2-124/H1.3-23) WAe001-A-82 (H1X-59, H1X59) WAe001-A-2C (SST-P2A-mCherry) WAe001-A-3S (H1-FCGR3A-EGFP) WAe001-A-1A (H1.2-13/H1.4-69) WAe001-A-1B (H1.2-124/H1.4-84) WAe001-A-2D (H1-FOXJ1-EGFP) WAe001-A-1C (H1.2-13/H1.5-23) |
| Last update | 12th December 2024 |
| User feedback | |
Provider |
|
| Generator |
Guangzhou Laboratory (GZL)
Contact:
Guangzhou Laboratory (GZL) |
| Owner | Guangzhou Laboratory (GZL) |
| Distributors | |
| Derivation country | China |
External Databases |
|
| BioSamples | SAMEA116095724 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
|
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has brother:
|
External Databases (Donor) |
|
| BioSamples | SAMEA7767418 |
Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | No |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | Consent documents may be obtained from NIH Registry (number 0043), or from WiCell |
| Alternative consent approval number | NIHhESC-10-0043 |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Please contact hPSCreg |
| Approval number | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | NIH Human Embryonic Stem Cell Registry |
| Approval number | NIHhESC-10-0043 |
hESC Derivation
| Date of derivation | 1998-01-01 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
| Derivation under GMP? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium | mTeSR™ 1 |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Present | Absent |
| mCpG | ||
| OCT4 | X |
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
The cell line can be differentiated into islet organiods.
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.