WA01, H1

General#

Cell Line

hPSCreg Name WAe001-A
Alternative name(s)
WA01, H1
Cell line type Human embryonic stem cell (hESC)
Last update 19th October 2018
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Provider

Generator WiCell Research Institute (WA)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA7768168
Cellosaurus CVCL_9771
NIHhESC NIHhESC-10-0043

General Information

Notes Detailed product information, including karyotypes, STR and HLA testing results can be found on WiCell website: https://www.wicell.org/home/stem-cells/catalog-of-stem-cell-lines/wa01.cmsx?closable=true
Publications
Projects
Is the cell line available in principle?
Yes
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7767418

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 1998-01-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Cell isolation Immunosurgery
Cell seeding Isolated ICM
Derivation under GMP?
No

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
LIF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TDGF1
Yes
Telomerase Activity
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 27

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)