Generation of a human embryonic stem cell (WAe001-A-47) with hVGLL4 doxycyclin-inducible expression by the PiggyBac transposon system


VGLL4 is a new component of the Hippo pathway and bind TEADs to compete with YAP, so as to inhibit tumor progression, but its role in stem cell and organ regeneration remains unclear. Using the PiggyBac transposon system, we generated a VGLL4 doxycycline-inducible expression human embryonic stem cell line (WAe001-A-47). The established hESC line retains its normal morphology and pluripotency markers with in vitro differentiation potential, as well as a normal karyotype. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Quan Y, Zhang T, Zhang H, Yang J, Jin P, Ma J, Fan Y, Fan J, Fan X, Gong Y, Li M, Wang Y
Journal Stem cell research
Publication Date 2020 Dec 24;50:102142
PubMed 33385792
DOI 10.1016/j.scr.2020.102142

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