Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1

Summary

ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endogenous regulatory machinery. The fluorescence engineered hESC clone maintains characteristic stem cell features and can differentiate to all three germ layers, serving as a unique tool to dissect the role of ATOX1 in various cellular processes. Copyright © 2019 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Wen MH, Xie X, Tu J, Lee DF, Chen TY
Journal Stem cell research
Publication Date 2019 Oct 16;41:101631
PubMed 31704540
DOI 10.1016/j.scr.2019.101631

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