Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endogenous regulatory machinery. The fluorescence engineered hESC clone maintains characteristic stem cell features and can differentiate to all three germ layers, serving as a unique tool to dissect the role of ATOX1 in various cellular processes. Copyright © 2019 The Author(s). Published by Elsevier B.V. All rights reserved.
|Authors||Wen MH, Xie X, Tu J, Lee DF, Chen TY|
|Journal||Stem cell research|
|Publication Date||2019 Dec;41:101631|