WAe001-A-29

General#

Cell Line

hPSCreg Name WAe001-A-29
Cell line type Human embryonic stem cell (hESC)
Last update 28th October 2019
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Provider

Generator University of Houston - Main Campus (UHMC)

External Databases

BioSamples SAMEA6260533

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7767418

Ethics#

Also have a look at the ethics information for the parental line WAe001-A .

hESC Derivation#

Date of derivation 1998-01-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line WAe001-A

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
copper ion binding
Synonyms
  • copper binding
Genetic modifications
Genetic modifications not related to a disease
mCherry (target)
Transgene expression
cytoband location: 5q33.1
One allele of this variant clone has mCherry insertion which will express functional mCherry-ATOX1 fusion protein. The other allele has a small deletion at 5-UTR of ATOX1, which silence the expression of untagged ATOX1 protein.
CRISPR-associated (CRISPR/Cas) System