WAe001-A-32

H1_RB1ex1_E9

General#

Cell Line

hPSCreg Name
WAe001-A-32
Alternative name(s)
H1_RB1ex1_E9
Cell line type Human embryonic stem cell (hESC)
Last update 24th February 2020
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Provider

Generator Universität Duisburg-Essen (UDE)
Distributors

External Databases

Cellosaurus CVCL_XC43
BioSamples SAMEA6877447
CLO CLO_0102914
Wikidata Q98134706

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7767418

Ethics#

Also have a look at the ethics information for the parental line WAe001-A .
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation#

Date of derivation 2016-11-09
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Embryo

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
TBXT
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Retinoblastoma
OMIM #180200
Synonyms
  • RB
  • retinoblastoma
  • RETINOBLASTOMA, MALIGNANT
  • Retinoblastoma
  • Retinoblastoma, NOS
show more synonyms
Genetic modifications
Gene knock-out
13q14.2
Cell line carries a 365 nucleotide deletion of RB1 exon1/promoter on one allele.
CRISPR-associated (CRISPR/Cas) System