WAe001-A-2B

H1-NSD2-/-

General

Cell Line

hPSCreg name WAe001-A-2B
Cite as:
WAe001-A-2B (RRID:CVCL_C9LE)
Alternative name(s)
H1-NSD2-/-
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe001-A
(WA01, H1)
WAe001-A-A
(H1X-10/H1.2-20)
WAe001-A-B
(H1X-59/H1.2-6)
WAe001-A-C
(H1X-10/H1.3-62)
WAe001-A-D
(H1X-59/H1.3-68)
WAe001-A-E
(H1X-10/H1.4-4)
WAe001-A-F
(H1X-59/H1.4-5)
WAe001-A-86
(H1.139, H1.1-39)
WAe001-A-72
(H1-FTO-Knockout-13)
WAe001-A-31
(H1_RB1ex1_D6)
WAe001-A-32
(H1_RB1ex1_E9)
WAe001-A-87
(H1.213, H1.2-13)
WAe001-A-G
(H1X-10/H1.5-1)
WAe001-A-88
(H1.2-124, H1.2124)
WAe001-A-1N
(H1-FIS1-KO, WA01-FIS1-KO)
WAe001-A-89
(H1.3-64, H1.364)
WAe001-A-H
(H1X-59/H1.5-33)
WAe001-A-1O
(WA01-MYB-KO, H1-MYB-KO)
WAe001-A-77
(H1 RYBP-FLAG-HA KI)
WAe001-A-90
(H1.3-191, H1.3191)
WAe001-A-I
(H1.0-29/H1.1-13)
WAe001-A-41
(H1-RNF2-KO-1#)
WAe001-A-J
(H1.0-102/H1.1-34)
WAe001-A-42
(H1-RNF2-KO-2#)
WAe001-A-K
(H1.0-29/H1.2-31)
WAe001-A-1
(H1.1, WA01.1)
WAe001-A-L
(H1.0-102/H1.2-6)
WAe001-A-M
(H1.0-29/H1.3-22)
WAe001-A-N
(H1.0-102/H1.3-90)
WAe001-A-91
(H1.4-126, H1.4126)
WAe001-A-O
(H1.0-29/H1.4-14)
WAe001-A-92
(H1.4-143, H1.4143)
WAe001-A-P
(H1.0-102/H1.4-19)
WAe001-A-93
(H1.5-23, H1.523)
WAe001-A-78
(H1X10, H1X-10)
WAe001-A-94
(H1.528, H1.5-28)
WAe001-A-Q
(H1.0-29/H1.5-6)
WAe001-A-73
(H1-GATAD2B-KO-79#)
WAe001-A-R
(H1.0-102/H1.5-44)
WAe001-A-10
(H1_RB1ex3_G3)
WAe001-A-74
(H1-GATAD2B-KO-81#)
WAe001-A-S
(H1.1-17/H1.2-11)
WAe001-A-11
(H1_RB1ex3_G4)
WAe001-A-T
(H1.1-39/H1.2-9)
WAe001-A-U
(H1.1-17/H1.4-18)
WAe001-A-V
(H1.1-39/H1.4-17)
Last update 28th August 2023
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Provider

Generator GIBH (CTU)
Contact:
GIBH (CTU)
Owner GIBH (CTU)
Distributors
Derivation country China

External Databases

BioSamples SAMEA114306601
Cellosaurus CVCL_C9LE
Wikidata Q123033810

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA7767418

Ethics

Also have a look at the ethics information for the parental line WAe001-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe001-A.

Culture Conditions

Surface coating Matrigel/Geltrex
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Differentiation Potency

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Gene knock-out
4p16.3
NSD2 gene homozygous knockout human embryonic stem cell lines using CRISPR/Cas9 system
CRISPR-associated (CRISPR/Cas) System