Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state


Biallelic inactivation of the retinoblastoma tumor suppressor gene (RB1) causes formation of retinoblastoma, a retinal eye tumor occurring in early childhood. Using the CRISPR/Cas9 nickase system, exon 1 of RB1 was deleted, including the RB1 promoter. As a result, sublines were generated carrying deletions of RB1 exon 1/promoter on one or both alleles. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Menges J, Cremanns M, Steenpass L
Journal Stem cell research
Publication Date 2019 Aug;39:101517
PubMed 31404748
DOI 10.1016/j.scr.2019.101517

Research Projects

Cell Lines