Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing
Summary
The human GLI3 protein has a dual function as a transcriptional activator or repressor of hedgehog signaling, depending on the proteolytic processing forms of GLI3. In this study, we established a compound heterozygous GLI3 mutant human embryonic stem cell line (WAe001-A-20) through CRISPR/Cas9 editing. The WAe001-A-20 cells carried two deletions on two different alleles of exon 2 of GLI3, respectively, which resulted in a frame shift and early termination in the translation of GLI3. Moreover, WAe001-A-20 maintains a normal karyotype, parental cell morphology, pluripotent phenotype and the ability to differentiate into three germ layers. Resource table. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wei R, Yuan F, Wu Y, Liu Y, You K, Yang Z, Chen Y, Getachew A, Wang N, Xu Y, Zhuang Y, Yang F, Li YX |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 Oct;32:139-144 |
| PubMed | 30278376 |
| DOI | 10.1016/j.scr.2018.09.010 |