Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing


The human GLI3 protein has a dual function as a transcriptional activator or repressor of hedgehog signaling, depending on the proteolytic processing forms of GLI3. In this study, we established a compound heterozygous GLI3 mutant human embryonic stem cell line (WAe001-A-20) through CRISPR/Cas9 editing. The WAe001-A-20 cells carried two deletions on two different alleles of exon 2 of GLI3, respectively, which resulted in a frame shift and early termination in the translation of GLI3. Moreover, WAe001-A-20 maintains a normal karyotype, parental cell morphology, pluripotent phenotype and the ability to differentiate into three germ layers. Resource table. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Wei R, Yuan F, Wu Y, Liu Y, You K, Yang Z, Chen Y, Getachew A, Wang N, Xu Y, Zhuang Y, Yang F, Li YX
Journal Stem cell research
Publication Date 2018 Oct;32:139-144
PubMed 30278376
DOI 10.1016/j.scr.2018.09.010

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