WAe001-A-56
General
Cell Line |
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hPSCreg Name | WAe001-A-56 |
Cell line type | Human embryonic stem cell (hESC) |
Last update | 8th June 2021 |
User feedback | |
Provider |
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Generator | University of Houston - Main Campus (UHMC) |
External Databases |
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BioSamples | SAMEA7710161 |
CLO | CLO_0103114 |
Cellosaurus | CVCL_A5QV |
Wikidata | Q107117410 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | male |
Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Family history | NO |
Is the medical history available upon request? | NO |
Is clinical information available? | NO |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
All cell lines of this donor's relatives |
Has brother:
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External Databases (Donor) |
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BioSamples | SAMEA7767418 |
Ethics
Also have a look at the ethics information for the parental line
WAe001-A
.
hESC Derivation
The source cell information can be found in the parental cell line
WAe001-A.
|
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzymatically
Accutase
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CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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NANOG |
Yes |
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SOX2 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
Genetic Modification
Genetic modifications not related to a disease |
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