WAe001-A-56
General#
Cell Line |
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| hPSCreg Name | WAe001-A-56 |
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 8th June 2021 |
| User feedback | |
Provider |
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| Generator | University of Houston - Main Campus (UHMC) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA7710161 |
| CLO | CLO_0103114 |
| Cellosaurus | CVCL_A5QV |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
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| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7767418 |
Ethics#
Also have a look at the ethics information for the parental line
WAe001-A
.
hESC Derivation#
| Date of derivation | 1998-01-01 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification#
| Genetic modifications not related to a disease |
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