Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1


Superoxide Dismutase 1 (SOD1) is an antioxidant enzyme that protects the cells from radical oxygen species. To study the behavior of endogenous SOD1 under a microscope, we genetically modified H1 human embryonic stem cells (hESCs) to express SOD1 fused with a SNAP-tag, a protein tag that can be covalently labeled with a variety of synthetic probes. The engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro, providing a versatile platform for imaging-based studies of SOD1. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Huang PS, Wen MH, Xie X, Xu A, Lee DF, Chen TY
Journal Stem cell research
Publication Date 2021 Jul;54:102415
PubMed 34118566
PubMed Central PMC8330834
DOI 10.1016/j.scr.2021.102415

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