Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
Superoxide Dismutase 1 (SOD1) is an antioxidant enzyme that protects the cells from radical oxygen species. To study the behavior of endogenous SOD1 under a microscope, we genetically modified H1 human embryonic stem cells (hESCs) to express SOD1 fused with a SNAP-tag, a protein tag that can be covalently labeled with a variety of synthetic probes. The engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro, providing a versatile platform for imaging-based studies of SOD1. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
|Authors||Huang PS, Wen MH, Xie X, Xu A, Lee DF, Chen TY|
|Journal||Stem cell research|
|Publication Date||2021 Jul;54:102415|