Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
Summary
Superoxide Dismutase 1 (SOD1) is an antioxidant enzyme that protects the cells from radical oxygen species. To study the behavior of endogenous SOD1 under a microscope, we genetically modified H1 human embryonic stem cells (hESCs) to express SOD1 fused with a SNAP-tag, a protein tag that can be covalently labeled with a variety of synthetic probes. The engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro, providing a versatile platform for imaging-based studies of SOD1. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Huang PS, Wen MH, Xie X, Xu A, Lee DF, Chen TY |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Jul;54:102415 |
| PubMed | 34118566 |
| PubMed Central | PMC8330834 |
| DOI | 10.1016/j.scr.2021.102415 |