Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9
Summary
The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Xu Y, Wu Y, Guo D, Gao G, Lai K, Yang F, Wang K, Wu H, Lai L, Li J, Xu K, Li YX |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2017 Jul;22:29-32 |
| PubMed | 28952928 |
| DOI | 10.1016/j.scr.2017.05.006 |