Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9


The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Xu Y, Wu Y, Guo D, Gao G, Lai K, Yang F, Wang K, Wu H, Lai L, Li J, Xu K, Li YX
Journal Stem cell research
Publication Date 2017 Jul;22:29-32
PubMed 28952928
DOI 10.1016/j.scr.2017.05.006

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