Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media


The derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) is a powerful tool to investigate early cardiogenesis and model diseases in vitro. Here, we present an optimized protocol to obtain contracting hPSCs-derived cardiomyocytes using a ready-to-use kit. We describe steps for hPSC culture and differentiation to cardiomyocytes including the identification of key parameters such as starting cell confluency and temperature. We then detail immunofluorescence, flow cytometry, and the quantification of cardiomyocytes' calcium spikes using live imaging. For complete details on the use and execution of this protocol, please refer to Astro et al.1. Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Authors Astro V, Ramirez-Calderon G, Adamo A
Journal STAR protocols
Publication Date 2023 Apr 13;4(2):102252
PubMed 37060558
PubMed Central PMC10140149
DOI 10.1016/j.xpro.2023.102252

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