KAUSTi011-B

iPSC-GLP1R-KO-H4

General

Cell Line

hPSCreg name KAUSTi011-B
Cite as:
KAUSTi011-B (RRID:CVCL_A1WH)
Alternative name(s)
iPSC-GLP1R-KO-H4
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 1st November 2020
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Provider

Generator King Abdullah University of Science and Technology (KAUST)
Owner King Abdullah University of Science and Technology (KAUST)
Distributors
Derivation country Saudi Arabia

External Databases

BioSamples SAMEA7679542
Cellosaurus CVCL_A1WH
Wikidata Q105509755

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 1-4
Ethnicity Saudi

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Epileptic encephalopathy and developmental delay.
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
6p21.2
NM_002062.3; c.402+3delG
Homozygous
Disease associated phenotypes
  • Epileptic encephalopathy and developmental delay.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7679541

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Dr. Fowzan S. Alkuraya: faikuraya@kfshrc.edu.sa / King Faisal Specialist Hospital and Research Centre
Alternatives to consent are available? No
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Patient Fibroblasts PT-15DG1507 was obtained from King Faisal Specialist Hospital and Research Center
Approval number RCGEN/342/40
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? KAUST Institutional Biosafety and Bioethics Committee
Approval number 17IBEC14
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Stemgent

hIPSC Derivation

General

Source cell line name Pt-15DG1507
Derived from same source line (potentially other lot and donor, see below):
Source cell type
Age of donor (at collection) 1-4
Passage number reprogrammed 4

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA, Small molecules, miRNA
mRNA
Small molecules

Other

Selection criteria for clones The emerging embryonic stem cell-like colonies were identified by distinct borders, bright centers, tight-packed cells and high nucleus to cytoplasm ratio
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
TrypLE
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-4
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Marker Expressed
Cytokeratin
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Marker Expressed
Desmin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Marker Expressed
S100
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)